Plasmid pBR322 has Pas I restriction enzyme site with in gene amp R that confers…
Biology · NEET · NTA Exams — Biotechnology: Principles and Processes
Plasmid pBR322 has Pas I restriction enzyme site with in gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for 
galactoside production and the recombinant plasmid is inserted in an E.coli strain
[NEET 2021]
galactoside production and the recombinant plasmid is inserted in an E.coli strain [NEET 2021]
- The transformed cells will have the ability to resist ampicillin as well as produce galactoside
- It will lead to lysis of host cell
- It will be able to produce a novel protein with dual ability
- It will not be able to confer ampicillin resistance to the host cell
Answer
(D) It will not be able to confer ampicillin resistance to the host cell
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