The following diagram shown restriction sites in E . coli cloning vector pBR322 . Find…

Biology · NEET · NTA Exams — Biotechnology: Principles and Processes

The following diagram shown restriction sites in E. coli cloning vector pBR322 . Find the role of ‘X’ and ‘Y’ genes
  [NCERT Pg. No. 169]
  [CBSE PMT (Pre) 2012; NEET 2024]

1. The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid
2. The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of plasmid
3. The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics
4. Gene ‘X’ is responsible for recognitions sites and ‘Y’ is responsible for antibiotic resistance

Answer

(B) The gene ‘ X ’ is responsible for controlling the copy number of the linked DNA and ‘ Y ’ for protein involved in the replication of plasmid

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